Antigen

Antigen Class

TFs and others

Antigen

CEBPB

Cell type

Cell type Class

Blood

Cell type

THP-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Leukemia cell line (THP1)

tissue

Leukemia cell line (THP1)

treatment

Cells cultured in arginine-free RPMI for 72h

cell type

Paediatric monocytic cell line derived from AML M5 with t(9;11)(q21;q23) translocation

chip antibody

Bethyl A302-738A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (transcription factors). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

37398717

Reads aligned (%)

97.3

Duplicates removed (%)

2.7

Number of peaks

56613 (qval < 1E-05)

hg19

Number of total reads

37398717

Reads aligned (%)

96.6

Duplicates removed (%)

2.9

Number of peaks

56282 (qval < 1E-05)